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. 1998 Feb;66(2):418–423. doi: 10.1128/iai.66.2.418-423.1998

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Copyright © 1998, American Society for Microbiology

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FIG. 1 — SDS-PAGE of ZZ-DTR and ZZ-DT_168–220 at different purification steps and stained with Coomassie blue R-250. Lanes 1 and 4, molecular mass markers (97.4, 66, 45, 31, 21.5, and 14.5 kDa); lane 2, crude extract of ZZ-DTR after induction with IPTG; lane 3, ZZ-DTR eluates from an IgG-Sepharose 4B column; lane 5, crude extract of ZZ-DT_168–220 after induction with IPTG; lane 6, ZZ-DT_168–220 after passage through an IgG-Sepharose 4B column; lane 7, ZZ-DT_168–220 after passage through a Mono S column.