Figure 1.

In utero electroporation of an anaesthetized mouse

(A) Workspace setup, cold source lamps, and surgery tools for in utero electroporation. Insert: Pulled borosilicate micropipettes stored on play dough in a 15-cm cell culture plate.

(B) Right before surgery, the mouse is anesthetized in an isoflurane chamber and the eyes are covered with Vidisic gel to prevent drying out of eyes.

(C) Fixation of the mouse with lab tape on its back on a heating pad with an isoflurane vaporizer nozzle fitting on the nose of the mouse.