Cortical cell preparation
(A) Workspace setup for cortical neuron preparation.
(B) Incision to remove the skin from the head.
(C) Removal of skull bone and meninges.
(D and E): Isolated mouse brain seen under the stereomicroscope: Bright field image shown in (D) and cortical region transfected with an eGFP-expressing plasmid under UV light seen in (E). Scale bar: 1 mm.