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Timetable summarizing the gradient for the basic reversed-phase (RP) chromatography, used for the fractionation of TMT-labeled peptides
| Time interval (min) | Gradient (percentage of buffer B) | Flow rate (nL/min) | |
|---|---|---|---|
| 0 | 3 | 200 | |
| 5 | 3 | 200 |  |
| 30 | 35 | 200 | |
| 30.1 | 98 | 200 | |
| 40 | 98 | 200 | |
| 40.1 | 3 | 200 | |
| 45 | 3 | 200 |
