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Timetable summarizing the gradient for the low pH RP chromatography, used online before MS3 analysis of TMT labeled peptides
| Time interval (min) | Gradient (percentage of buffer B) | Flow rate (nL/min) | |
|---|---|---|---|
| 0 | 2 | 300 | |
| 10 | 2 | 300 |  |
| 70 | 30 | 300 | |
| 70.1 | 90 | 300 | |
| 75 | 90 | 300 | |
| 75.1 | 2 | 300 | |
| 90 | 2 | 300 |
