Trypsin stock solution (see Step 3.a)
| Reagent | Final concentration |
|---|---|
| Triethyl ammonium bicarbonate (TEAB) | 100 mM |
| Sodium deoxycholate (SDC) | 1% |
| Trypsin | 0.5 mg/mL |
Dissolve in ddH2O, pH 8.5, store at −20°C and do not store for more than 2 weeks.
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