TABLE 2.

PCR primers

Target	Sequencea	Annealing temp (°C)	References
vsp family	5′-AAGTCTGACGGAACAGTACT-3′	50	7, this study
	5′-GTTATTTTGAGAAGGTTTTTC-3′
vlp family
α subfamily	5′-CTAATGATAGGWTGTGGACAAC-3′	55	7, this study
	5′-CTCAAGAACATTCTTTACTGTC-3′
β subfamily	5′-GTGATGCATTAGGATTTAATGC-3′	55	7, this study
	5′-CCTAATACCTTATTTACWGCAC-3′
γ subfamily	5′-CTAGTGACCCAATTGCTAATGT-3′	55	7, this study
	5′-TCTAGTGCTTTAGTAATTGCAC-3′
δ subfamily	5′-ATACTAAGAAAAGTGATATAGG-3′	50	7, this study
	5′-CCATTGCTCGCAGTGCAATGCC-3′
Flagellin gene	5′-ACATATTCAGATGCAGACAGAGGT-3′	60	39, this study
	5′-GCAATCATTGCCATTGCAGATTGT-3′

^aW, A or T.