Table 2.

Recommended histological, immunohistochemical and molecular workup of liver nodule(s) in patients with congenital portosystemic shunts.

	Recommendations
Recommended routine stains	Haematoxylin/eosin Reticulin stain
Recommended immunohistochemistry	Glutamine synthetase β-catenin SAA (serum amyloid A) CRP (C-reactive protein) CD34 Glypican-3LFABP (liver-fatty acid binding protein)
Recommended minimal molecular work-up	β-catenin activation (CTNNB1 exon3, 7/8) TERT promoter mutation
Recommended sample size	Needle gauge should be sufficient to provide adequate diagnostic yield according to local laboratory standards.