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. 2023 Dec 23;79:101860. doi: 10.1016/j.molmet.2023.101860

Figure 4.

Figure 4

Effects of maternal overnutrition and anti-diabetic treatments on AgRP and α-MSH fiber density in the anterior portion of the paraventricular nucleus of the hypothalamus (PVHant) in P21 offspring. (A, A′) Representative images of AgRP projections and their quantification in the anterior paraventricular nucleus of the hypothalamus (PVH) of P21 male offspring. (B, B′) Representative images of AgRP projections and their quantification in the anterior paraventricular nucleus of the hypothalamus (PVH) of P21 female offspring. (C, C′) Representative images of α-MSH projections and their quantification in the anterior paraventricular nucleus of the hypothalamus (PVH) of P21 male offspring. (D, D′) Representative images of α-MSH projections and their quantification in the anterior paraventricular nucleus of the hypothalamus (PVH) of P21 female offspring. Data are presented as mean ± SEM with single data points. Statistical analyses were performed using two-way ANOVA. Scale bar is 100 μm. 3V: third ventricle; PVH: paraventricular nucleus of the hypothalamus; CD/CD: gestational and lactational control diet-exposed; CD/HFD: gestational control diet and lactational high-fat diet-exposed; HFD/HFD: gestational and lactational high-fat diet-exposed.