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. 2023 Dec 23;79:101860. doi: 10.1016/j.molmet.2023.101860

Figure 5.

Figure 5

Metformin does not have an impact on the cell number of the two main arcuate neuronal populations. (A, A′) Representative images of TdTomato-labelled AgRP neurons and their quantification in the mid-portion of the ARH. (B, B′) Representative images of TdTomato-labelled POMC neurons and their quantification in the mid-portion of the ARH. Data are presented as mean ± SEM with single data points. Blue dots represent males and orange dots represent females. Statistical analyses were performed using two-way ANOVA. Scale bar is 100 μm. 3V: third ventricle; ARH: arcuate nucleus of the hypothalamus; CD/CD: gestational and lactational control diet-exposed; CD/HFD: gestational control diet and lactational high-fat diet-exposed; HFD/HFD: gestational and lactational high-fat diet-exposed.