Table 4.
Two-way ANOVA results for Figure 2.
| Panel | Source of variation | F (DFn, DFd) | P value |
|---|---|---|---|
| A) Body weight vehicle group males | Diet | F (2, 36) = 5.34 | P = 0.009 |
| Time | F (3.59, 129.3) = 1635 | P < 0.001 | |
| Diet x Time | F (34,612) = 15.37 | P < 0.001 | |
| B) Body weight insulin-exposed males | Diet | F (2, 34) = 11.03 | P < 0.001 |
| Time | F (4.12, 140) = 1279 | P < 0.001 | |
| Diet x Time | F (34, 578) = 8.73 | P < 0.001 | |
| C) Body weight metformin-treated males | Diet | F (2, 31) = 6.17 | P = 0.006 |
| Time | F (4.21, 130.6) = 656.90 | P < 0.001 | |
| Diet x Time | F (34, 527) = 6.49 | P < 0.001 | |
| D) Body weight P16 males | Diet | F (2, 156) = 6.81 | P = 0.001 |
| Treatment | F (2, 156) = 15.20 | P < 0.001 | |
| Diet x Treatment | F (4, 156) = 9.30 | P < 0.001 | |
| E) Body weight change males | Diet | F (2, 158) = 9.88 | P < 0.001 |
| Treatment | F (2, 158) = 25.20 | P < 0.001 | |
| Diet x Treatment | F (4, 158) = 4.92 | P < 0.001 | |
| F) Glycemia P16 males | Diet | F (2, 94) = 0.87 | P = 0.422 |
| Metformin | F (1, 94) = 10.21 | P = 0.002 | |
| Diet x Metformin | F (2, 94) = 0.22 | P = 0.804 | |
| G) Insulin levels P16 males | Diet | F (2, 31) = 4.78 | P = 0.016 |
| Metformin | F (1, 31) = 3.76 | P = 0.062 | |
| Diet x Metformin | F (2, 31) = 0.01 | P = 0.989 | |
| H) Leptin levels P16 males | Diet | F (2, 34) = 10.45 | P < 0.001 |
| Metformin | F (1, 34) = 2.11 | P = 0.155 | |
| Diet x Metformin | F (2, 34) = 2.25 | P = 0.121 | |
| I) Ghrelin levels P16 males | Diet | F (2, 33) = 2.13 | P = 0.135 |
| Metformin | F (1, 33) = 10.32 | P = 0.003 | |
| Diet x Metformin | F (2, 33) = 0.54 | P = 0.586 | |
| J) GDF15 levels P16 males | Diet | F (2, 23) = 3.11 | P = 0.064 |
| Metformin | F (1, 23) = 0.66 | P = 0.423 | |
| Diet x Metformin | F (2, 23) = 0.99 | P = 0.384 | |
| K) Body weight vehicle group females | Diet | F (2, 37) = 11.44 | P < 0.001 |
| Time | F (2.47, 91.48) = 1295 | P < 0.001 | |
| Diet x Time | F (34, 629) = 12.23 | P < 0.001 | |
| L) Body weight insulin-exposed females | Diet | F (2, 30) = 16.48 | P < 0.001 |
| Time | F (3.91, 117.40) = 920.40 | P < 0.001 | |
| Diet x Time | F (34, 510) = 8.82 | P < 0.001 | |
| M) Body weight metformin-treated females | Diet | F (2, 36) = 6.99 | P = 0.003 |
| Time | F (3.15, 113.3) = 471.40 | P < 0.001 | |
| Diet x Time | F (34, 612) = 5.42 | P < 0.001 | |
| N) Body weight P16 females | Diet | F (2, 160) = 11.40 | P < 0.001 |
| Treatment | F (2, 160) = 30.10 | P < 0.001 | |
| Diet x Treatment | F (4, 160) = 8.71 | P < 0.001 | |
| O) Body weight change females | Diet | F (2, 157) = 5.74 | P = 0.004 |
| Treatment | F (2, 157) = 37.50 | P < 0.001 | |
| Diet x Treatment | F (4, 157) = 6.05 | P < 0.001 | |
| P) Glycemia P16 females | Diet | F (2, 76) = 3.86 | P = 0.025 |
| Metformin | F (1, 76) = 3.50 | P = 0.065 | |
| Diet x Metformin | F (2, 76) = 1.93 | P = 0.152 | |
| Q) Insulin levels P16 females | Diet | F (2, 28) = 3.96 | P = 0.031 |
| Metformin | F (1, 28) = 0.06 | P = 0.812 | |
| Diet x Metformin | F (2, 28) = 0.36 | P = 0.702 | |
| R) Leptin levels P16 females | Diet | F (2, 28) = 9.24 | P < 0.001 |
| Metformin | F (1, 28) = 0.04 | P = 0.838 | |
| Diet x Metformin | F (2, 28) = 0.69 | P = 0.511 | |
| S) Ghrelin levels P16 females | Diet | F (2, 31) = 0.41 | P = 0.666 |
| Metformin | F (1, 31) = 9.98 | P = 0.004 | |
| Diet x Metformin | F (2, 31) = 0.39 | P = 0.682 | |
| T) GDF15 levels P16 females | Diet | F (2, 26) = 15.49 | P < 0.001 |
| Metformin | F (1, 26) = 0.21 | P = 0.650 | |
| Diet x Metformin | F (2, 26) = 1.23 | P = 0.310 |