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. 2024 Jan 17;31:8. doi: 10.1186/s12929-024-00995-x

Table 2.

Mutations incorporated into infectious clones by site-directed mutagenesis

Clone Backbone Nucleotide
5459
Nucleotide
8677
Amino acid nsP3-471 Amino acid
E2-55
CHIKV-BP ···C··· ···G··· Serine (Ser) Glycine (Gly)
CHIKV-SP ···T··· ···A··· Proline (Pro) Arginine (Arg)
CHIKV-m1 CHIKV-BP ···C··· ···A··· Serine (Ser) Arginine (Arg)
CHIKV-m2 CHIKV-SP ···T··· ···G··· Proline (Pro) Glycine (Gly)
CHIKV-m3 CHIKV-BP ···T··· ···G··· Proline (Pro) Glycine (Gly)
CHIKV-m4 CHIKV-SP ···C··· ···A··· Serine (Ser) Arginine (Arg)