FIG. 4.

Oral immunization with SREHP-H generates ASC in spleen and MLN cells producing IgA and IgG antiamebic antibodies. (A and B) Numbers of IgA and IgG ASC, respectively, from spleen cells obtained from mice immunized with SREHP-H, SREHP-H/CT, MBP-H/CT, or CT alone that produce antiamebic antibodies (EH), anti-CTB antibodies (CTB), or anti-FCS (FCS) antibodies. (C and D) Numbers of IgA and IgG ASC, respectively, in MLN cells obtained from mice immunized with SREHP-H, SREHP-H/CT, MBP-H/CT, or CT alone producing antiamebic antibodies, anti-CTB antibodies, and anti-FCS antibodies. Bars show the mean values ± the standard errors of the means (error bars) for groups of five to seven mice. An asterisk over the bar indicates that the mean value was significantly higher (P < 0.05) than the corresponding FCS (control) value.