Table 1.
Sequences, annealing temperatures, and product size of PCR primers used in the current studya
| Target gene | Primers | Nucleotide sequences (5′–3′) | Annealing temp (°C) | Product length |
|---|---|---|---|---|
| 18S rRNA (HVR-I) | Forward (RH5401) | AAACATGAAACCGCGGAAAG | 52 | 862 bp |
| Reverse (RH5402) | CATTCTTGGCAAATGCTTTCG | |||
| Sequencing (RH5403) | AGCTGGAATTACCGCGGCTG | |||
| 18S rRNA (HVR-IV) | Forward (18SP4F) | GCGAAAGCATTTGCCAA | 57 | 712 bp |
| Reverse (18SPCR) | ACGGCCGGTGTGTAC | |||
| Sequencing (ZS6269) | GTGGTGCATGGCCGTTC | |||
| cox-1 | Forward (ZS6985) | GGTGGTTTTGGTAATTGAATG | 47 | 837 bp |
| Reverse (ZS6986) | ACCAGTYAAACCACCAATAGTAA | |||
| Sequencing (ZS6990) | GGTTGATAAACTATAACAGTACC |
aAll primers were taken from Zhou et. al. [19]