(A) Eupenifeldin is cytotoxic in 2D foci assay. Cell lines were treated for 8 h with either eupenifeldin (10 nM), paclitaxel (10 nM), or solvent control. Letters represent statistically significantly different groups with p < 0.05. (B) Representative 2D foci assay images. (C) A hollow fiber assay demonstrated decreased OVCAR3 cell survival after eupenifeldin treatment. Mice were treated with vehicle control (0.2% DMSO/PEG/H2O) (n = 8), chemotherapeutic control (paclitaxel, 5 mg/kg) (n = 6), or eupenifeldin (0.5 mg/kg)(n = 8) once per day for 4 days. Data represented as mean ± SEM and analyzed with one-way ANOVA using PRISM and statistical analysis conducted using Dunnett’s multiple comparison (* P = < 0.05).