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(A, B) The sensitivity of OVCAR3 cells to ferroptosis induction with RSL3 and IKE was validated. (C) The cytotoxicity induced by eupenifeldin treatment was not reversed by ferrostatin-1 addition, implicating that a nonferroptotic death pathway is involved. (D) The ability of eupenifeldin to induce lipid peroxidation was tested, and no increase in lipid peroxidation was observed.
