SDS-PAGE and western blot analysis of plant-produced RSV-F Fc fusion protein. SDS-PAGE stained with Instant Blue™ under non-reducing (a) and reducing condition (b) whereas, lane M, Protein ladder; lane 1, purified RSV F-Native Fc fusion protein; lane 2, purified SC-TM Fc fusion protein. Western blot analysis under non-reducing conditions (c) and reducing condition (d), the membrane was probed with anti-human IgG Fc HRP. The arrowhead indicates the major band.