Table 3.
Efficiency of ccpA deletion in C. symbiosum.
| Plasmids | Elements | Results (M/P/W/T)a | Efficiencyb (%) | Conjugation efficiency |
|---|---|---|---|---|
| Control | Cas12a + crRNA | none | – | – |
| pMTLcas-ΔccpA | Cas12a + crRNA + Homologous arms |
4/1/27/32 | 15.6 % | 2.48 ± 0.28 × 10−8 |
a
M: number of colonies that harbored mixed wild-type and genedeleted cells; P: number of colonies that harbored pure gene-deleted cells; W: number of colonies that harbored pure wild-type cells; T: total number of colonies used for PCR screening.
b
Efficiency: probability of deletion events occurring, calculated as (M + P)/T × 100 %.