FIG. 3.

LPS binding to differentiated THP-1 cells in the presence of rhLBP. As described in Materials and Methods, cells were incubated with 1 μg of FITC-labeled LPS per ml in the presence of 0.4% BSA (bar 1), 1.5 μg of rhLBP per ml (bar 2), 1.5 μg of rhLBP per ml after preincubation of cells with anti-CD14 monoclonal antibody (bar 3), or 1.5 μg of rhLBP per ml and 20 μg of hLf per ml added at the same time (bar 4). The mean fluorescence intensity was determined. The results (means ± standard errors) were calculated from four separate experiments.