FIG. 5.

Time-dependent inhibition of rhLBP-mediated binding of LPS to differentiated THP-1 cells in the presence of hLf. The binding of FITC-labeled LPS was studied, as described in Materials and Methods, in the presence of rhLBP (1.5 μg/ml) and three concentrations of hLf added at various intervals: 5 μg/ml (filled bars), 10 μg/ml (hatched bars), and 20 μg/ml (stippled bars). hLf was added to LPS at the same time as rhLBP (A), 30 min before rhLBP (B), or 30 min after rhLBP (C). Results are percentages of the total LPS bound to cells in the presence of rhLBP alone. Data are expressed as the means ± standard errors from three replicates.