FIG. 6.

Inhibition of LPS binding to differentiated THP-1 cells by rhLf and mutated rhLfs in the presence of rhLBP. Cells were incubated for 1 h at 4°C simultaneously with 1 μg of FITC-labeled LPS per ml, 1.5 μg of rhLBP per ml, and increasing concentrations of rhLf (•), EGS-rhLf (▴), or G4R-rhLf (○). Results are percentages of total LPS bound to cells in the presence of rhLBP alone. Each point represents the mean ± standard error from four experiments.