Fig. 1. A Key Use Case for Multi-Organ Imaging: Studying Mechanisms of Nociception and Pain.

Peripheral nociceptive input that drives pain engages a wide range of dynamic body systems. Integrated activity spans multiple organs, including skin, dorsal root ganglia (DRG), spinal cord and brain, and depends critically on a broad array of networks, including neural, vascular and immune signaling. State-of-the-art approaches for studying sensory information processing typically rely on in vivo imaging in one organ, and focuses on neurons. In the present work, we expand the in vivo methodological toolbox by developing procedures for multi-organ in vivo studies.