Fig. 7. Bioluminescence (BL) Imaging Was Conducted in Anesthetized Animals With Direct Cortical Luciferin Application and In Awake Behaving Animals With Systemic Injection.

(a) We performed acute BL imaging under isoflurane anesthesia with direct cortical administration of luciferin (Coelenterazine). The same preparation was used for imaging either using an emCCD camera or the BLmini. During the experiments testing novel BL calcium indicators (e.g., CaBLAM (APD3A6)) we stimulated vibrissae using a piezoelectric element. (b) For awake BL and FL (e.g., jRGeCO) imaging we administered luciferin intraperitonially (IP) before headpost-restraining the animal on a running wheel. We imaged BL and FL through the same cranial window sequentially to compare relative activation profiles.