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[Preprint]. 2023 Dec 27:2023.12.25.573323. [Version 1] doi: 10.1101/2023.12.25.573323

Fig. 9. UPRIM Spinal Cord Implants are Viable for Single Cell GCaMP Imaging.

Fig. 9

(a) Individual cell GCaMP6f calcium responses to hind paw pinch were imaged under anesthesia in an animal implanted with spinal cord UPRIM via surgical procedure # IV and using Miniscope v4. Calcium indicator was expressed via AAV2/9-CaMKIIa-GCaMP6f-P2A-nls-dTomato. (b) A pinch response map as the average of multiple trials, showing activation in multiple distinct neurons (pink, green and blue circles). (c) Time series show changes in signal for each color-matched cell. Grey shadings highlight the pinching time intervals, arrows emphasize responses that differed between 3 cells.