Fig. 9. UPRIM Spinal Cord Implants are Viable for Single Cell GCaMP Imaging.
(a) Individual cell GCaMP6f calcium responses to hind paw pinch were imaged under anesthesia in an animal implanted with spinal cord UPRIM via surgical procedure # IV and using Miniscope v4. Calcium indicator was expressed via AAV2/9-CaMKIIa-GCaMP6f-P2A-nls-dTomato. (b) A pinch response map as the average of multiple trials, showing activation in multiple distinct neurons (pink, green and blue circles). (c) Time series show changes in signal for each color-matched cell. Grey shadings highlight the pinching time intervals, arrows emphasize responses that differed between 3 cells.