Key Reagents
| Item | Company/Source | Identifier |
|---|---|---|
| Plasmids | ||
| Tet-FLAG-FOXMl | Addgene | 68810 |
| psPAX2 | Addgene | 12260 |
| pMD2.G | Addgene | 12259 |
| CENP-C-LAP | Iain Cheeseman | Gift |
| C-Terminal-LAP (EV) | Todd Stukenberg | Gift |
| siRNAs | ||
| ON-TARGETplus Human CENPF siRNA SMARTpool | Dharmacon | L-003253-00-0010 |
| ON-TARGETplus Human FOXM1 siRNA SMARTpool | Dharmacon | L-009762-00-0005 |
| Silencer™ Select Negative Control No. 2 siRNA | Invitrogen | 4390846 |
| Antibodies | ||
| CENPF (Mouse) | BD Biosciences | 610768 |
| CENPF (Rabbit) | Cell Signaling Technology | 58982 |
| FOXM1 (Rabbit) | Cell Signaling Technologies | 5436 |
| α-Tubulin (Mouse) | DSHB | 12G10 |
| ACA (Human) | Antibodies Incorporated | 15–235 |
| LIN54 (Rabbit) | Bethyl Laboratories | A303-799A |
| MYBL2 (Mouse) | Millipore | MABE886 |
| MYBL2 (Rabbit) | Proteintech | 18896-1-AP |
| GAPDH (Rabbit) | Bethyl Laboratories | A300-641A |
| CENPA (Rabbit) | Cell Signaling Technology | 2186 |
| Rabbit IgG | Cell Signaling Technology | 2729 |
| DaR-FITC | Jackson ImmunoResearch | 711-095-152 |
| GαM-Cy3 | Jackson ImmunoResearch | 115-165-146 |
| DαH-Cy3 | Jackson ImmunoResearch | 709-165-149 |
| GaM-FITC | Jackson ImmunoResearch | 115-095-003 |
| FLAG-HRP (M2) | Sigma | A8592 |
| GαR-HRP | Jackson ImmunoResearch | 111-035-003 |
| GαM-HRP | Jackson ImmunoResearch | 115-035-003 |
| qPCR Primers | ||
| Primer Name | Forward | Reverse |
| CENPA | CTCGTGGTGTGGACTTCAAT | CTGCATGTAAGGTGAGGAGATAG |
| CENPF | GAAGCAGCTCTGTGTCTTACA | CTTGGTTTGTCCTGGCTATCT |
| FOXM1 | CAATTGCCCGAGCACTTGGAATCA | TCCTCAGCTAGCAGCACCTTG |
| CENPC | CTCCACCTCATTCGTGTCCTC | CCCTGCCTTTCTTGGTATTGTAA |
| CCNB1 promoter | CGCGATCGCCCTGGAAACGCA | CCCAGCAGAAACCAACAGCCGT |
| AURKB promoter | GGGGTCCAAGGCACTGCTAC | GGGGCGGGAGATTTGAAAAG |