Table 2.

Microfluidic Circuit Components (Scale Bar: 1 mm)

Component	Image	Function	Circuit Symbol
Lifting gate valves Micropump		To pump reagents including cell culture media, normal bath, or calcium indicator to cells
Quake Valve		To create a chemica gradient during the delivery of cytokines cells
Microfluidic rectifier Diodic valve		Ensure backflow elimination and stabilization of flow within the cell culture chamber
Gate Valve		Allow separation of downstream pump from the cell culture and cytokine chambers
Cytokine and cell culture chambers		Storage of cytokines and cells
CAM-μTAS		Fully automated microfluidic system to monitor calcium dynamics in microglia following a cytokines concentration gradient-based delivery