Fig. 2. The backward reaction (B) of CSSC back to the CSH precursor. (A) The addition of DTT causes the breakdown of CSSC fibers back into CSH with the appearance of transient stacked nanorods. (B) CryoEM image of fibers before the addition of DTT. (C) CryoEM image at 1579 s, 75 s after the addition of DTT, fibers break up into nanorods with some of these rods showing 2-D stacking. (D) CryoEM image at 2110 s, most fibers have disassembled as the solutions return to a sol state, however a few fibers/nanorods can be seen in samples by cryoEM indicating that a small number of fibers could be kinetically trapped. Scale bars are 200 nm.