Experimental parameters for the forward (assembly) and backward (disassembly) processes as well as the synchronous (transient self-assembly) process for CSH/CSSC system. All experiments were conducted at a pH of 6.0. B is performed from a completed sample of F and the collection time builds off F.
| Runa | Initial materials | #CryoEM time points | Collection time range |
|---|---|---|---|
| F | 10 mM CSH, 10 mM H2O2 | 4 | 8–1500 s |
| B | 5 mM CSSC (F after 1500 s), 200 mM DTT | 4 | 1575–3375 s (& 21 days) |
| S | 10 mM CSH, 150 mM H2O2, 200 mM DTT | 22 | 8–24 000 s (& 19 days) |
a
F = forward, B = backward, S = synchronous.