Chemical reactions of CSH/CSSC systems used to simulate the stochastic kinetics.

Number	Reaction	Forward rate constant	Reverse rate constant
1	CSH + OH− ⇌ CS− + H2O	k CSH = 10pH−pKakCSHr	k CSH = rD(CSH)/V
2	CS− + H2O2 → CSOH + OH−	k 3 = 25 M−1 s−1
3	CS− + CSOH → CSSCsol + OH−	k 4 = 720 M−1 s−1 [4]
4	CSSCsol ⇌ CSSCfib	k 7 = 1.46 × 10−17 M−1 s−1	k sp = 1000 M−1 s−1
5	DTT + OH− ⇌ DTT− + H2O	k DTT = 10pH−pKakDTTr	k DTT = rD(DTT)/V
6	DTT− + CSSCsol → DTTSC + CS−	k 6 = 5 M−1 s−1
7	DTT− + CSOH → DTTSC + OH−	k 5 = 10 M−1 s−1
8	DTTSC → DTTcyc + CSH	k d = 2.83 × 10−17 M−1 s−1
9	DTT− + H2O2 → DTTOH + OH−	k 1 = 0.0046 M−1 s−1
10	DTTOH → DTTcyc + OH−	k d = 2.83 × 10−17 M−1 s−1
11	2CSSCsol → 2CSSCfib	k 7 = 1.46 × 10−17 M−1 s−1