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Effect of conjugation to KLH on the immunogenicity of nonimmunogenic peptides. Hsd:ND4 mice were immunized with either 30 μg of free peptide or 50 μg of conjugated peptide on days 0, 30, and 60. Additional animals served as controls and were immunized with the carrier molecule KLH, Plague Vaccine USP, or the recombinant V protein. The mice were bled on day 70, and the sera were analyzed by ELISA for the presence of V-specific antibodies. aa, amino acids.
