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. 2024 Jan 12;15(1):2302246. doi: 10.1080/21655979.2024.2302246

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© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Dose–response diagrams show that all investigated anti-CD40 antibodies induce IL8 production in the presence of FcγRIIB expressing cells. — FcγR binding boosts anti-CD40 antibody-triggered IL8 production. (a) Specific binding of IgG1, IgG2, IgG4 and IgG1(N297A) variants of G28.5 to murine FcγRIIB. (b) CD40-responsive U2OS were challenged with IgG1, IgG2, IgG4 and IgG1(N297A) variants of the indicated anti-CD40 antibodies along with HEK293 cells transfected with empty vector (EV) or expression plasmids encoding FcγRIIB. Next day, cell supernatants were analyzed for IL8 production as readout of CD40 activation. (c) Co-cultures of U2OS and murine A20 cells, which endogenously express FcγRs, were stimulated with the αCD40(APX)-IgG antibodies and the next day, IL8 production was again determined by ELISA.