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. 2024 Jan 12;15(1):2302246. doi: 10.1080/21655979.2024.2302246

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© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Western Blot panels show that all investigated anti-CD40 antibodies induce TRAF1 and p100 processing in the presence of FcγRIIB expressing cells. — FcγR binding enhances anti-CD40 antibody-induced p100 processing. (a) U2OS cells were stimulated overnight with HEK293 cells transfected with empty vector (EV) or an expression plasmid encoding FcγRIIB and 150 ng/ml of the indicated antibodies or remained without antibody treatment (no ab). Total cell lysates were analyzed for p100 processing. (b) Human U2OS cells were stimulated overnight with murine L929 cells not expressing FcγRs or murine A20 cells expressing endogenously FcγRIIB and again 150 ng/ml of the indicated antibodies. L929 and A20 cells were also antibody-treated in the absence of U2OS cells (-) as control for bands no derived of the human U2OS cells. Total cell lysates were again analyzed for p100 processing.