. Author manuscript; available in PMC: 2024 Jan 17.

Published in final edited form as: J Invertebr Pathol. 2022 Aug 3;194:107804. doi: 10.1016/j.jip.2022.107804

Table 2:

Fungal isolate source table. Sources, culture media and extraction methods for fungal species used in this study.

Ascosphaera species	Strain^a	Isolation Source^b	DNA Extraction method
aggregata	USDA-ARS PIBMSR wild2	Single spore isolate from Megachile rotundata: Logan, UT; Cultured in lab on V-8 media	PureGene Salty
atra	ARSEF 5147	Isolated from honey in Apis mellifera colony: Waroona, Western Australia; Cultured in lab on SDA	PureGene Salty
pollenicola	ATCC 62712	Isolated from pollen stores of Megachile rotundata: Western Canada; Cultured in lab on PDA	PureGene Salty
acerosa	ATCC 201316	Isolated from Megachile rotundata: Lethbridge, Alberta; Cultured in lab on SDA	MoBio Ultra Clean Plant DNA Kit

ARSEF: Agricultural Research Service Collection of Entomopathogenic Fungi, Ithaca, New York; ATCC: American Type Culture Collection, Manassas, Virginia; USDA-ARS PIBMSR: Pollinating Insect-Biology, Management, Systematics Research: Logan, Utah.

SDA: Saborard Dextrose Agar; PDA: Potato Dextrose Agar; V8: Modified V8 agar (James and Buckner 2004)