FIG. 2.

Amino acid sequences and reactivities with antibodies of both region 1 from CopB of four different M. catarrhalis strains and synthetic peptides derived from these sequences. The numbers (i.e., 275 to 300) above the region 1 sequences indicate the amino acid positions in the intact CopB protein. The numbers (i.e., 1 to 26) above the R1 synthetic peptide indicate residue positions in the R1 peptide and its derivatives. Shading indicates residues differing from those in O35E. The reactivities of MAb 10F3, GST-26 antiserum, and GST antiserum with the CopB proteins of these strains in Western blot analysis are indicated on the right; these two antisera were diluted 1:100 for use in this assay. The reactivities of the synthetic peptides with these same antibody preparations in the ELISA are indicated on the right opposite these peptides. The asterisk indicates that the ELISA with MAb 10F3 was performed after adsorption of the peptides to the microtiter wells in the presence of glutaraldehyde. The ELISA readings (optical densities at 410 nm) were recorded after 30 min as described in Materials and Methods.