FIG. 5.

Western blot analysis of the reactivities of MAbs and polyclonal serum antibodies with outer membrane proteins of M. catarrhalis strains, the GST-26 fusion protein, and GST alone. Proteins present in outer membrane vesicles from strains O35E (lane 1), O12E (lane 2), TTA24 (lane 3), and O46E (lane 4) and purified GST-26 (lane 5) and GST (lane 6) were resolved by SDS-PAGE, transferred to nitrocellulose, and probed with MAb 10F3 (A) or with mouse GST-26 antiserum (dilution 1:1,000) (B). The CopB protein from strain O35E gave rise to a doublet reactive with MAb 10F3 in this experiment. The positions of the molecular mass markers (in kilodaltons) are indicated to the left of each panel.