TABLE 4.
Number of intracellular bacteria within the HeLa cell monolayers at 1, 6, and 12 h postinfection and 6 h postinfection in the presence of cefotaxime
| Strain | CFU/ml (log10)a per HeLa cell monolayer at indicated time (h) postinfection
|
|||
|---|---|---|---|---|
| 1 | 6 | 6 + cefotaxime | 12 | |
| M90T | 330.0 ± 23.6 | 1,116.7 ± 236.29 | 0.016 ± 0.005 | 12.975 ± 4.582 |
| ZB2106 (M90T ΔpurE) | 466.7 ± 48.5 | 893.3 ± 52.4c | 0.109 ± 0.032 | 15.250 ± 2.986 |
| ZB2100 (M90T thyA::Tn10) | 33.33 ± 3.0 | 0.044 ± 0.03 | 0.107 ± 0.024 | NGb |
| ZB2104 (M90T ΔdapB-Km) | 86.67 ± 8.5 | 2.173 ± 1.514 | 2.303 ± 0.135 | NG |
| ZB2111 (M90T aroB-Ap) | 300.0 ± 75.9 | 916.7 ± 80.71 | 0.036 ± 0.006 | 6.680 ± 2.523 |
| ZB2102 (M90T aroC::Tn10) | 216.7 ± 25.5 | 383.3 ± 99.62 | 4.360 ± 1.122 | NG |
| ZB2103 (M90T aroD::Tn10) | 94.33 ± 49.3 | 620.0 ± 45.6 | 0.230 ± 0.055 | 3.225 ± 1.330d |
| ZB2112 (M90T ΔpurE aroB-Ap) | 390.0 ± 11.5 | 526.7 ± 29.14 | 0.221 ± 0.020 | 4.510 ± 1.957 |
| ZB2108 (M90T ΔpurE aroC::Tn10) | 633.3 ± 101.5 | 1,187.0 ± 232.0 | 2.175 ± 0.902 | NG |
| ZB2109 (M90T ΔpurE aroD::Tn10) | 360.0 ± 101.5 | 860.0 ± 79.05 | 1.735 ± 0.201 | NG |
| ZB2107 (M90T ΔpurE thyA::Tn10) | 251.0 ± 114.2 | 0.084 ± 0.011 | 0.211 ± 0.022 | NG |
a
Mean ± standard deviation from three separate invasion assays.
b
NG, no growth was observed.
c
P = 0.0081 (Student’s t test).
d
P = 0.0145 (Student’s t test).