Extended Data Fig. 2. Mre11, but not ATM, is required for quiescence and ISG induction in Myc^OEp53^−/− pMMECs.

a, Western blot confirmation of ATM, Mre11, cGAS, and Actin in Myc^OEp53^−/− pMMECs expressing the indicated sgRNAs, demonstrating consistent findings across three independent replicates. b, Representative images for 24-hours EdU pulse-chase quiescence assay in Myc^OEp53^−/− pMMECs. Scale bar, 20μm. c, Quantification of EdU negative percentage. n = 1,500 (sgControl, sgMre11, sgcGas and sgAtm) independent cells. ****, p < 0.0001. Representative of 2 independent biological experiments; d, 8 days after infection with each lentivirus, qRT-PCR was performed to normalize gene expression levels for interferon-stimulated genes IFIT1, with mRNA levels normalized to β-actin mRNA levels. N = 3 (sgCon, sgMre11, sgcGas and sgAtm) independent samples. NT vs sgControl; p = 0.0002. sgControl vs sgMre11; p = 0.0047, sgcGas; p = 0.0052. n = 3 independent biological experiments;. Data are mean ± SEM. Unless otherwise specified, statistical analyses were determined by one-way ANOVA followed by Sidak’s multiple comparison post-test using a two-tailed test: *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Source Data