Extended Data Fig. 4. Time-lapse microscopy of cGAS recruitment to cytoplasmic dsDNA in WT and Mre11-mutant cells.

a, Time-lapse microscopy of WT and sgMRE11 MDA-MB-231 cells expressing RFP-cGAS after transfection with 4 μg/mL Alexa 488-ISD90. Images were captured with a Nikon fluorescence microscope, and the time stamp is relative to transfection. White arrows indicate co-localization of RFP-cGAS foci and Alexa 488-ISD90 foci. Scale bar, 20 μm.