Extended Data Fig. 6. Detail of the structure of GFP containing a β-amino acid.

Details of structure GFP(150(S)β³mBrF)–His₆ (PDB code 8OVY). a, Detail of protein chain at position 150. The acquired structure for GFP(150(S)β³mBrF)–His₆ (in yellow) is superimposed with the structure of wt GFP used for refinement (PDB: 2B3P), showing the kink in the backbone induced by the β³-amino acid. b, Detail of hydrogen bond network in the beta-barrel at position 150. The GFP(150(S)β³mBrF)–His₆ structure (yellow) is superimposed to the structure of WT GFP (PDB: 2B3P). The kink induced by incorporation of (S)β³mBrF (13) affects the local hydrogen bond at that position, however the remaining contacts of the corresponding beta-strand remain intact.