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. 2023 Dec 20;625(7995):578–584. doi: 10.1038/s41586-023-06856-1

Extended Data Fig. 1. Purification of PfRCR-Cy.003 complex and cryo-EM processing statistics.

Extended Data Fig. 1

a, Size exclusion chromatography profile and b, Reducing SDS-PAGE gel for the reconstituted PfRCR-Cy.003 complex, representative of five independent experiments. The fraction labelled with an asterisk corresponds to the region highlighted on the size exclusion chromatography profile in panel a containing the PfRCR-Cy.003 complex. Both full-length PfRH5 and truncated PfRH5 (PfRH5*) were observed in the purified complex. c, Gold-standard Fourier shell correlation curves of the consensus PfRCR-Cy.003 reconstruction. d, Particle view distribution of the consensus PfRCR-Cy.003 reconstruction. e, Map regions corresponding to PfRIPR in the consensus PfRCR-Cy.003 reconstruction and following local refinement, showing improvement in map quality. Both maps are coloured by local resolution and post-processed with DeepEMhancer. f, Consensus map of the PfCyRPA-PfRIPR-Cy.003 (CR3) complex, post-processed with DeepEMhancer, and coloured by local resolution as in (e). g, Gold-standard Fourier shell correlation curves of the consensus PfCyRPA-PfRIPR-Cy.003 reconstruction. h, Particle view distribution of the consensus PfCyRPA-PfRIPR-Cy.003 reconstruction.

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