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. 2024 Jan 17;10:34. doi: 10.1038/s41420-023-01794-3

Fig. 3. Knockdown of HMGB1 suppresses NMDAR activation-induced ferroptosis.

Fig. 3

A Representative image of immunostaining of HMGB1 in the presence of GLU or NMDA in HUVECs. B Representative cell viability of the control siRNA or HMGB1 siRNA-transfected HUVECs in the presence of GLU or NMDA with MTT assay (n = 5). C Protein expression levels and (D) quantitation of HMGB1, GPX4, and SLC7A1 in the control siRNA or HMGB1 siRNA-transfected HUVECs in the presence of GLU or NMDA (n = 5). E MDA content, (F) iron content, and (G) GSH levels in the control siRNA or HMGB1 siRNA-transfected HUVECs in the presence of GLU or NMDA (n = 5). H Scheme of the mechanism of NMDAR inhibitor MK-801 in VEC ferroptosis. *P < 0.05, ***P < 0.001 vs control siRNA; ###P < 0.001 vs control siRNA + GLU, &P < 0.05 and &&&P < 0.001 vs control siRNA + NMDA.