Fig. 11. Hepatic malondialdehyde (MDA) deposition following the polymicrobial infection is significantly reduced in mice treated with nisin.

MDA in liver tissue was quantified to evaluate the ability of nisin to modulate lipid peroxidation due to oxidative stress in the histological sections stained by immunohistochemistry (A) (Scale bar: 200 μm). Four different fields (100× magnification) were randomly selected on the images of three tissue sections per mouse specimen (n = 3 per group), and area of brown-stained MDA deposition (B) was measured using ImageJ analysis software. The data in the bar graphs are shown as means ± standard deviation. *p < 0.05 between groups and **p < 0.01 between groups with Dunn’s test.