Figure 2.

TNF, IL1α, and C1q knockout reduces GFAP+ cell number in the peri-infarct area 7- and 30-days post ischemic stroke. Low- (5x) and high-magnification (40x) representative images of GFAP+ cells in the infarct and peri-infarct area for wildtype (WT) and Tnf^−/−Il1a^−/−C1q^−/− triple knockout (TKO) mice 1- (A), 7-days post-dMCAO (C) and 30-days post-photothrombotic stroke (E). (B) No difference was found in GFAP+ cells in the peri-infarct area 1 day after ischemia between the WT and TKO mice, t₍₂₉₎ = 1.29, p = 0.104 post-dMCAO. (D) TKO mice had significantly reduced GFAP+ cells in the peri-infarct area than WT mice 7 days post-dMCAO, t₍₁₂₎ = 2.64, p = 0.011. (F) TKO mice had significantly reduced GFAP+ cells in the peri-infarct area than WT mice 30 days post-photothrombotic stroke, t₍₁₄₎ = 5.03, p < 0.0001. * p < 0.05, ** p < 0.001, *** p < 0.0001. GFAP; glial fibrillary acidic protein, TKO, Tnf^−/−Il1a^−/−C1q^−/− triple knockout mouse; WT, wild type; DV, dorsoventral; ML, mediolateral.