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. 2024 Jan 4;5:1269601. doi: 10.3389/ftox.2023.1269601

TABLE 2.

Experimental setup of the Muta-ChromoPlate™ Assay with S9 activation for T98 and T100 bacterial strains.

Treatment plate (1–12) Standard Sample Water Reaction mix S9 mix Bacteria (5 µL)
Blank (sterility check) - 15.5 0 2.5 2.0 -
Background - - 15.5 2.5 2.0 +
Positive control 0.1 - 15.5 2.5 2.0 +
WWTP effluent I (with S9) - 15.5 0 2.5 2.0 +
WWTP effluent I - 15.5 0 2.5 0.0 +
WWTP effluent II - 3.0 12.5 2.5 2.0 +
Tap water I (with S9) - 15.5 0 2.5 2.0 +
Tap water I - 15.5 0 2.5 0.0 +
Tap water II - 3.0 12.5 2.5 2.0 +
4-CP
Bottled water A-I (with S9) - 15.5 0 2.5 2.0 +
Bottled water A-I - 15.5 0 2.5 0.0 +
Bottled water A-II - 15.5 0 2.5 0.0 +
2,4-DCP
Bottled water B-I (with S9) - 3.0 12.5 2.5 2.0 +
Bottled water B-I - 15.5 0 2.5 2.0 +
Bottled water B-II - 3.0 12.5 2.5 2.0 +

Bold values indicate the sectioning of the tables.