TABLE 2.
Experimental setup of the Muta-ChromoPlate™ Assay with S9 activation for T98 and T100 bacterial strains.
| Treatment plate (1–12) | Standard | Sample | Water | Reaction mix | S9 mix | Bacteria (5 µL) |
|---|---|---|---|---|---|---|
| Blank (sterility check) | - | 15.5 | 0 | 2.5 | 2.0 | - |
| Background | - | - | 15.5 | 2.5 | 2.0 | + |
| Positive control | 0.1 | - | 15.5 | 2.5 | 2.0 | + |
| WWTP effluent I (with S9) | - | 15.5 | 0 | 2.5 | 2.0 | + |
| WWTP effluent I | - | 15.5 | 0 | 2.5 | 0.0 | + |
| WWTP effluent II | - | 3.0 | 12.5 | 2.5 | 2.0 | + |
| Tap water I (with S9) | - | 15.5 | 0 | 2.5 | 2.0 | + |
| Tap water I | - | 15.5 | 0 | 2.5 | 0.0 | + |
| Tap water II | - | 3.0 | 12.5 | 2.5 | 2.0 | + |
| 4-CP | ||||||
| Bottled water A-I (with S9) | - | 15.5 | 0 | 2.5 | 2.0 | + |
| Bottled water A-I | - | 15.5 | 0 | 2.5 | 0.0 | + |
| Bottled water A-II | - | 15.5 | 0 | 2.5 | 0.0 | + |
| 2,4-DCP | ||||||
| Bottled water B-I (with S9) | - | 3.0 | 12.5 | 2.5 | 2.0 | + |
| Bottled water B-I | - | 15.5 | 0 | 2.5 | 2.0 | + |
| Bottled water B-II | - | 3.0 | 12.5 | 2.5 | 2.0 | + |
Bold values indicate the sectioning of the tables.