TABLE 2.

The number of safe, broad, and effective (SBE) gRNAs found by the NDNF pipeline.

Cas	PAM motif	Safe	Broad	Effective	SBE	Unique sites
ASp2Cas12l	CCY	17	255	6,696	1	1
AacCas12b	TTN	6	1,047	8,946	1	1
AsCas12a	TTTN	14	329	8,427	3	2
Asp2Cas12l	CCY	11	267	6,742	2	1
BthCas12b	ATTN	12	135	7,379	8	2
Cas12c2	TN	1	5,207	9,606	1	1
Cas12d	TA	7	850	8,809	0
Cas12e	TTCN	19	83	6,313	0
Cas12h1	RTR	1	615	8,471	1	1
Cas12j	TBN	12	3,534	9,333	0
CasY	TA	9	871	8,785	1	1
CjeCas9	NNNRYAC	7	11	5,695	0
Cpf1-TATV	TATV	41	21	6,981	6	3
Cpf1-TTTN	TTTN	132	105	8,573	19	4
Cpf1-TTTR	TTTR	48	35	6,710	0
Cpf1-TTTV	TTTV	55	43	7,464	10	2
Cpf1-TYCV	TYCV	142	50	6,337	2	2
ErCas12a	YTTN	17	603	8,310	1	1
FnCas12a	TTV	8	529	7,900	1	1
FnCas9-RHA	YG	8	810	8,150	0
GeoCas9	NNNNCRAA	0	1	4,754	0
LbCas12a_Cpf1	TTTN	212	1,051	41,970	26	4
Nme1Cas9	NNNNGATT	6	0	4,590	0
OspCas12c	TG	11	595	7,912	0
SaCas9-KKH-A	NNARRT	43	22	6,942	1	1
SaCas9-KKH-C	NNCRRT	33	2	6,043	0
SaCas9-KKH-N	NNNRRT	236	227	8,808	37	9
SaCas9-KKH-T	NNTRRT	45	17	7,102	6	3
SaCas9-WT	NNGRRT	103	260	42,779	9	2
SauriCas9	NNGG	27	600	7,841	5	4
SauriCas9-KKH	NNRG	11	1,936	8,506	1	1
ScCas9	NNG	11	2,773	9,022	2	2
SpCas9-EQR	NGAG	62	22	5,033	0
SpCas9-NG	NG	365	1,291	8,879	41	29
SpCas9-VQR	NGA	164	230	7,140	19	7
SpCas9-VRER	NGCG	6	4	3,852	0
SpCas9-WT	NGG	633	2,027	46,571	73	14
SpRY	NRN	378	5,582	10,169	24	22
SpaCas9	NNGYRA	3	11	6,290	1	1
St1Cas9	NNAGAAW	27	15	4,716	11	1
Un1Cas12f1	TTTR	16	110	6,724	0
spaCas9	NNGYRA	3	12	6,381	1	1
xCas	NGN	7	2,904	8,862	0