FIGURE 5.

Impact of Boswellin® Super (BSR) on the modulation and induction of transcellular LM formation in co-incubations of human neutrophils and platelets. Co-incubations of neutrophils and platelets (10⁷ and 25 × 10⁷ cells/mL, respectively) were pretreated with the indicated concentrations of BSR, AKBA (10 µM), or vehicle (0.2% ethanol) for 30 min and then stimulated with 1% SACM for 90 min at 37°C. Formed LM were isolated from the supernatants and analyzed by UPLC-MS/MS. (A) Heatmap showing the fold changes in LM formation (logarithmic scale) for BSR- or AKBA-versus vehicle-pretreated cells stimulated with 1% SACM, n = 3, separate donors. (B,C) Co-incubations of neutrophils and platelets (10⁷ and 25 × 10⁷ cells/mL, respectively) with the indicated concentrations of BSR, AKBA (10 µM), or vehicle (0.2% ethanol) for 180 min at 37°C. Formed LM were isolated from the supernatants and analyzed by UPLC-MS/MS. (B) Heatmap showing the fold increase in LM formation for BSR- or AKBA- versus vehicle-treated cells, n = 3, separate donors. (C) Data are given as pg/co-incubation, means ± S.E.M., n = 3, separate donors. For statistical analysis, data were log-transformed, one-way analysis of variance (ANOVA) with Dunnett’s multiple comparison test, *p < 0.05, **p < 0.01, and ***p < 0.001 against vehicle.