TABLE 1.

Modulation of LM pathways in innate immune cells by BSR. M1- and M2-MDMs (10⁶), neutrophils (5 × 10⁶), and platelets (5 × 10⁸) were preincubated with 50 μg/mL BSR or vehicle (0.2% ethanol) for 30 min and then stimulated with 1% SACM for 90 min at 37°C. Formed LM were isolated from the supernatants and analyzed by UPLC-MS/MS and are given in pg (PUFAs in ng) per 10⁶ M1- and M2-MDMs, 5 × 10⁶ neutrophils, and 5 × 10⁷ platelets, n = 3.

	pg	M1-MDM			M2-MDM			Neutrophils			Platelets
		Veh.	BSR	Fold	Veh.	BSR	Fold	Veh.	BSR	Fold	Veh.	BSR	Fold
SPMs	RvD5	≤3	≤3	1.0	3,664 ± 404	6,627 ± 649	1.8	27 ± 13	≤3	0.1	≤3	≤3	1.0
	RvE4	≤3	≤3	1.0	127 ± 30	382 ± 64	3.0	≤3	≤3	1.0	10 ± 3.6	9.1 ± 1.7	0.9
	LXA4	≤3	≤3	1.0	35 ± 13	110 ± 45	3.1	≤3	≤3	1.0	≤3	≤3	1.0
12-LOX products	14-HDHA	116 ± 6.2	136 ± 16	1.2	4,675 ± 867	12,337 ± 1,596	2.6	332 ± 68	638 ± 164	1.9	7,678 ± 1,702	6,760 ± 1,472	0.9
	12-HEPE	49 ± 2.5	58 ± 15	1.2	720 ± 115	1,539 ± 37	2.1	240 ± 35	673 ± 209	2.8	4,912 ± 1,395	5,519 ± 1,381	1.1
	12-HETE	300 ± 26	483 ± 83	1.6	3,929 ± 535	12,169 ± 865	3.1	5,740 ± 238	14,173 ± 3,299	2.5	62,466 ± 7,754	87,756 ± 14,716	1.4
15-LOX products	17-HDHA	550 ± 35	806 ± 123	1.5	21,044 ± 6,850	25,084 ± 5,658	1.2	108 ± 59	250 ± 91	2.3	≤3	≤3	1.0
	15-HEPE	80 ± 10	111 ± 22	1.4	4,255 ± 1,081	5,422 ± 552	1.3	21 ± 11	59 ± 27	2.8	≤3	≤3	1.0
	15-HETE	1,346 ± 462	2,188 ± 758	1.6	31,450 ± 6,777	45,198 ± 3,687	1.4	338 ± 138	1,278 ± 458	3.8	40 ± 7.7	51 ± 16	1.3
5-LOX products	7-HDHA	182 ± 29	120 ± 20	0.7	267 ± 32	817 ± 127	3.1	71 ± 12	22 ± 5.1	0.3	≤3	≤3	1.0
	5-HEPE	164 ± 46	41 ± 8.9	0.2	72 ± 12	69 ± 14	0.9	187 ± 20	19 ± 6.9	0.1	≤3	≤3	1.0
	5-HETE	4,654 ± 1,485	884 ± 211	0.2	273 ± 89	538 ± 82	2.0	1,901 ± 346	194 ± 44	0.1	2.9 ± 0.5	3.6 ± 1.0	1.2
	LTB4	1,892 ± 1,055	262 ± 113	0.1	164 ± 48	147 ± 22	0.9	2,200 ± 944	53 ± 14	0.0	≤3	≤3	1.0
COX products	PGE2	5,701 ± 4,832	5,483 ± 4,447	1.0	423 ± 47	952 ± 43	2.3	98 ± 5.0	422 ± 96	4.3	59 ± 7.2	279 ± 42	4.7
	PGD2	166 ± 57	326 ± 172	2.0	205 ± 28	711 ± 121	3.5	12 ± 2.5	57 ± 12	4.6	22 ± 3.2	67 ± 13	3.0
	PGF2α	385 ± 116	375 ± 126	1.0	101 ± 26	91 ± 18	0.9	13 ± 0.8	13 ± 4.0	1.0	24 ± 3.2	109 ± 27	4.5
	TXB2	7,985 ± 3,404	5,845 ± 3,124	0.7	10,041 ± 1,650	2,981 ± 503	0.3	575 ± 77	792 ± 234	1.4	5,979 ± 654	14,681 ± 3,446	2.5
ng
PUFAs	AA	1,521 ± 1,375	1,451 ± 51	1.0	955 ± 3,561	872 ± 354	0.9	294 ± 606	719 ± 27	2.4	11 ± 22	19 ± 4.5	1.7
	EPA	390 ± 354	1,027 ± 43	2.6	208 ± 819	386 ± 170	1.9	11 ± 23	106 ± 15	9.2	1.9 ± 3.7	4.1 ± 0.8	2.2
	DHA	926 ± 393	578 ± 64	0.6	430 ± 1,832	439 ± 250	1.0	65 ± 92	124 ± 10	1.9	1.5 ± 2.8	1.3 ± 0.5	0.9