TABLE 2.
Induction of LM pathways in innate immune cells by BSR. M1- and M2-MDMs (106) neutrophils (5 × 106), and platelets (5 × 108) were pre-incubated with 50 μg/mL BSR or vehicle (0.2% ethanol) for 180 min at 37°C. Formed LM were isolated from the supernatants and analyzed by UPLC-MS/MS and are given in pg (PUFAs in ng) per 106 M1- and M2-MDMs, 5 × 106 neutrophils, and 5 × 107 platelets, n = 3.
| pg | M1-MDM | M2-MDM | Neutrophils | Platelets | |||||||||
| Veh. | BSR | Fold | Veh. | BSR | Fold | Veh. | BSR | Fold | Veh. | BSR | Fold | ||
| SPMs | RvD5 | ≤3 | ≤3 | 1.0 | 3.6 ± 2.1 | 625 ± 242 | 173 | ≤3 | ≤3 | 1.0 | ≤3 | ≤3 | 1.0 |
| RvE4 | ≤3 | ≤3 | 1.0 | ≤3 | ≤3 | 1.0 | ≤3 | ≤3 | 1.0 | ≤3 | ≤3 | 1.0 | |
| LXA4 | ≤3 | ≤3 | 1.0 | ≤3 | ≤3 | 1.0 | ≤3 | ≤3 | 1.0 | ≤3 | ≤3 | 1.0 | |
| 12-LOX products | 14-HDHA | 11 ± 6 | 80 ± 12 | 7.5 | 10 ± 4.6 | 1,706 ± 607 | 171 | 8.9 ± 3.5 | 161 ± 23 | 18 | 869 ± 269 | 6,160 ± 1,144 | 7.1 |
| 12-HEPE | 8 ± 8 | 39 ± 17 | 5.0 | 5.0 ± 2.6 | 189 ± 72 | 38 | ≤3 | 284 ± 73 | 95 | 679 ± 237 | 7,587 ± 1,577 | 11 | |
| 12-HETE | 64 ± 35 | 307 ± 137 | 4.8 | 21 ± 3.5 | 908 ± 336 | 44 | 88 ± 19 | 6,253 ± 871 | 71 | 10,191 ± 3,074 | 1,07,653 ± 11,637 | 11 | |
| 15-LOX products | 17-HDHA | ≤3 | 454 ± 73 | 151 | 36 ± 18 | 4,003 ± 1,113 | 112 | ≤3 | 87 ± 32 | 29 | ≤3 | ≤3 | 1.0 |
| 15-HEPE | 9 ± 9.0 | 50 ± 8.5 | 5.5 | 10 ± 5.9 | 857 ± 273 | 85 | ≤3 | 34 ± 20 | 11 | ≤3 | ≤3 | 1.0 | |
| 15-HETE | 38 ± 19 | 692 ± 99 | 18 | 63 ± 10 | 8,376 ± 2,461 | 134 | 16 ± 4.1 | 840 ± 339 | 53 | 22 ± 6.5 | 32 ± 3.3 | 1.5 | |
| 5-LOX products | 7-HDHA | 17 ± 17 | 55 ± 3.3 | 3.3 | ≤3 | 92 ± 24 | 31 | ≤3 | ≤3 | 1.0 | ≤3 | ≤3 | 1.0 |
| 5-HEPE | 4.6 ± 2.0 | 14 ± 1.5 | 3.0 | ≤3 | ≤3 | 1.0 | ≤3 | 2.3 ± 0.5 | 0.8 | ≤3 | ≤3 | 1.0 | |
| 5-HETE | 28 ± 10 | 211 ± 41 | 7.6 | 9.3 ± 3.1 | 52 ± 10 | 5.6 | 19 ± 4.7 | 54 ± 16 | 2.8 | 3.1 ± 0.7 | 3.0 ± 0.5 | 1.0 | |
| LTB4 | ≤3 | 48 ± 9.2 | 16 | ≤3 | 8.4 ± 4.3 | 2.8 | 4.5 ± 1.2 | 6.5 ± 0.7 | 1.4 | ≤3 | ≤3 | 1.0 | |
| COX products | PGE2 | 620 ± 440 | 1,762 ± 1,367 | 2.8 | 11 ± 2.9 | 112 ± 35 | 10 | 9.1 ± 1.6 | 384 ± 107 | 42 | 32 ± 6.4 | 349 ± 24 | 11 |
| PGD2 | ≤3 | ≤3 | 1.0 | 5.9 ± 3.2 | 150 ± 47 | 25 | ≤3 | 47 ± 12.2 | 16 | 9.5 ± 1.2 | 70 ± 6.4 | 7.4 | |
| PGF2α | 118 ± 30 | 195 ± 41 | 1.7 | 9.4 ± 3.2 | 9.3 ± 2.3 | 1.0 | ≤3 | 11 ± 2.7 | 3.7 | 12 ± 0.8 | 124 ± 17 | 10 | |
| TXB2 | 1,734 ± 511 | 2,653 ± 875 | 1.5 | 545 ± 186 | 222 ± 31 | 0.4 | 149 ± 43 | 745 ± 195 | 5.0 | 4,498 ± 285 | 15,434 ± 2,673 | 3.4 | |
| ng | |||||||||||||
| PUFAs | AA | 28 ± 5.1 | 472 ± 102 | 17 | 14 ± 1.9 | 112 ± 41 | 8.0 | 11 ± 2.1 | 190 ± 31 | 18 | 3.7 ± 0.5 | 11 ± 1.8 | 2.9 |
| EPA | 7.0 ± 0.6 | 354 ± 60 | 51 | 3.6 ± 1.2 | 39 ± 12 | 11 | 0.6 ± 0.1 | 32 ± 4.8 | 56 | 0.5 ± 0.1 | 2.6 ± 0.3 | 5.0 | |
| DHA | 38 ± 10 | 248 ± 51 | 6.5 | 12 ± 2 | 64 ± 26 | 5.2 | 2.6 ± 0.5 | 14 ± 2.4 | 5.5 | 1.0 ± 0.2 | 1.0 ± 0.5 | 1.0 | |