TABLE 3.
Lectins used in competitive inhibition binding studies
| Lectin source | Common name (lectin) | Oligosaccharide recognitiona | Reference |
|---|---|---|---|
| Canavalia ensiformis | Jack bean (concanavalin A) | Branched N-linked hexasaccharides | 14 |
| Limulus polyphemus | Horseshoe crab (L. polyphemus agglutinin) | NAcNeu, NeuGNAc | 25 |
| Maackia amurensis | Maackia (M. amurensis agglutinin) | NAcNeu-α(2,3)Gal | 32 |
| Triticum vulgare | Wheat germ (wheat germ agglutinin) | Manβ(1,4)GlcNAc-β(1,4)GlcNAc | 34 |
| NAcNeu |
a
NAcNeu, N-acetylneuraminic acid; NeuGNAc, N-acetylglycolylneuraminic acid; Gal, galactose; Man, mannose; GlcNAc, N-acetylglucosamine. Agents were prepared to pH 7.2 in HBSS and added to macrophage host cells for 1 h, cells were washed to remove excess, and assays were performed in a competitive fashion.