Fig. 2.

Co-immunoprecipitation of Bcl-XL, IRS-1 Ser312, and Beclin-1. Two different amounts (100 and 150 μg) of protein lysate (indicated above each lane) from infected and uninfected cells were immunoprecipitated with antibody against Bcl-XL (a), IRS-1 Ser312 (b), and Beclin-1 (c). The lysates were fractionated on a 10% PAGE and probed individually with all three antibodies. Respective cell lysates were included in each gel as a control for a direct Western blot. Specific protein bands, as well as those for IgG, are marked. The IRS-1 Ser12 and Beclin-1 bands in (b, c) were published previously but presented again for comparison [12]. The reduced intensity of the band for Bcl-XL in b is probably due to the loss of precipitated material in one lane during the course of processing of the sample.